Published July 28, 2019
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1
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NMR spectra of media and vesicle fluid of in vitro grown Echinococcus multilocularis metacestodes
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Description
The metabolic fingerprint of in vitro grown E. multilocularis metacestodes was determined. E. multilocularis metacestodes were grown in rat hepatoma-cell pre-conditioned culture medium (cDMEM) for 72 hours at 37°C. After this incubation period, parasite-interacted media (vcDMEM, n=10) and control incubated media (ccDMEM, n=10) were analyzed by nuclear magnetic resonance spectroscopy (NMR: 1D 1H, 2D 1H-1H, and 2D 1H-13C Bruker DRX 600MHz). In addition, also vesicle fluid (VF, n=5) samples from metacestodes were analyzed after 72 hours of incubation by NMR accordingly.
Table of contents
# The names are corresponding to the .zip file in the archive # vcDMEM_ccDMEM-VF-1H-1D-NMR.zip 1D 1H NOESY # folder sample name 10 VF_1 20 VF_2 30 VF_3 40 VF_4 50 VF_5 610 vcDMEM_7 620 ccDMEM_3 640 vcDMEM_5 650 vcDMEM_6 680 ccDMEM_2 700 ccDMEM_9 720 vcDMEM_2 730 ccDMEM_1 740 ccDMEM_8 750 vcDMEM_1 800 ccDMEM_7 820 ccDMEM_4 850 vcDMEM_4 870 vcDMEM_8 900 vcDMEM_9 920 vcDMEM_3 970 vcDMEM_10 990 ccDMEM_6 1000 ccDMEM_5 1030 ccDMEM_10 # vcDMEM_ccDMEM-COSY-STOCSY-HSQC-Jres-2D-NMR.zip 2D # folder sample name 10 ccDMEM NOESY 11 ccDMEM COSY 12 ccDMEM TOCSY 13 ccDMEM Jres 14 ccDMEM HSQC 20 vcDMEM NOESY 21 vcDMEM COSY 22 vcDMEM TOCSY 23 vcDMEM Jres 24 vcDMEM HSQC # VF-COSY-STOCSY-HSQC-Jres-2D-NMR.zip 2D # folder sample name 1 VF NOESY 2 VF COSY 3 VF TOCSY 4 VF Jres 5 VF HSQCMethods
400 µl of each sample were mixed with 250 µl sodium phosphate buffer (D2O:H2O, v/v, 9:1; pH=7.4; 0.01% trimethylsilylpropanoic acid (TSP); 3 mM sodium-azide), vortexed and centrifuged. 600 µl of each sample were transferred into a 5 mm diameter NMR tube. 1H NMR spectroscopy was recorded at 300 K on a Bruker DRX 600MHz NMR spectrometer (Bruker Biospin; Rheinstetten, Germany) at 600.29 MHz. Each spectrum was acquired with 2.73 sec acquisition time for 128 scans (spectral width = 20.2 ppm) with a standard pulse sequence (recycle delay-90°−t1-90°-tm-90°-acquire free induction decay (FID)). Two-dimensional (2D) 1H-1H correlation spectroscopy (COSY), 1H-1H total correlation spectroscopy (TOCSY), and 1H-13C heteronuclear single quantum coherence (HSQC) NMR were acquired from selected samples from each sample group (32 scans).Files
vcDMEM_ccDMEM-COSY-STOCSY-HSQC-Jres-2D-NMR.zip
Files
(257.7 MB)
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Checksum: md5:291e40ac2f01208d1aeccf2b77ba4b42
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Additional details
Identifiers
- b2rec
- cf3ac1ee15a743df99f044befea73f68