Published September 13, 2019
| Version
v1
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Open
NMR spectra of urine from mice infected with Echinococcus multilocularis, and respective control urines from non-infected mice
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Description
Effects of Echinococcus multilocularis infection on the metabolic composition of mouse urine were assessed. 10 mice were perorally (intragastic gavage) infected with 400 E. multilocularis eggs each. 12 control mice were mock infected. Urine samples were collected 11 weeks post infection. After this incubation period urine samples were analyzed by nuclear magnetic resonance spectroscopy (NMR: 1D 1H).
Table of contents
# The names are corresponding to the .zip file in the archive # Mouse_urine-EMultilocularis_infected-and_control _1H-1D-NMR.zip # Folder name group urine volume collected [µL] 10 infected 113 20 infected 97 30 infected 47 40 infected 40 50 infected 76 60 infected 44 70 infected 51 80 infected 134 90 infected 42 100 infected 43 110 control 83 120 control 119 130 control 75 140 control 49 150 control 77 160 control 81 170 control 72 180 control 47 190 control 56 200 control 84 210 control 40 220 control 40Methods
Urine samples were centrifuged at maximum speed for 10 min at 4 °C. 35 µl of each sample supernatant were mixed with 35 µl sodium phosphate buffer (D2O:H2O, v/v, 9:1; pH=7.4; 0.01% trimethylsilylpropanoic acid (TSP); 3 mM sodium-azide), vortexed and centrifuged. 60 µl of each sample were transferred into a 1.7 mm diameter NMR tube. 1H NMR spectroscopy was recorded at 300 K on a Bruker DRX 600MHz NMR spectrometer (Bruker Biospin; Rheinstetten, Germany) at 600.29 MHz. Each spectrum was acquired with 2.73 sec acquisition time for 128 scans (spectral width = 20.2 ppm) with a standard pulse sequence (recycle delay-90°−t1-90°-tm-90°-acquire free induction decay (FID)).Files
Mouse_urine-EMultilocularis_infected-and_control _1H-1D-NMR.zip
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(23.5 MB)
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Checksum: md5:8431ea413a15635c76c4e7f0604362ac
PID: http://hdl.handle.net/11304/c35d9ce1-7e79-4b79-993d-9c2167795dba |
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Additional details
Identifiers
- b2rec
- 92571ee0eb434a8ea71a26f9b3db92d3
BBMRI metadata
- Disease
- Alveolar echinococossis