Published November 23, 2025 | Version 1.0
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Camel Bones as a Source of Fat: Optimization of Extraction Methods and Fatty Acid Composition Analysis

  • 1. Laboratory of Studies of Ionized & Reactive Media (EMIR LR20ES08), IPEIM, University of Monastir, B.P 56, 5000, Tunisia
  • 2. ISSAT of Sousse, Energy Department, University of Sousse, 4003, Sousse, Tunisia
  • 3. Laboratory of Valorization and promotion of Saharan resources (VPRS), Department of Chemistry, University of Ouargla, P.O. Box 511, 30000, Ouargla , Algeria

Description

Acta Chim. Slov. 2025, 72, 748–760. DOI: 10.17344/acsi.2024.9089

Abstract

This study explores the viability of using camel bones, an abundant by-product in North Africa and the Middle East, as a novel source of fat for biodiesel production. A moist-heat extraction process using a pressure cooker was employed to extract fat from both hollow and flat bones. The initial phase of the study optimized temperature (40-100 °C) and duration (0.5–5 hours) using ordinary water, confirming that fat yield increased with both parameters. A subsequent phase significantly enhanced extraction efficiency by introducing two key optimizations: grinding the bones to increase surface area and using distilled water to eliminate ionic interference. This approach achieved a peak yield of 26.69% (by bone mass) for hollow bones at 100 °C after 6 hours. Compositional analysis indicated a predominance of saturated fatty acids. The findings confirm that camel bones are a promising fat source for industrial applications, such as biodiesel production via transesterification, with an optimal extraction window of 3 to 5 hours identified for an efficient process.

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Acta Chim. Slov. 2025, 72, 748–760
DOI
10.17344/acsi.2024.9089

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Journal article: 10.17344/acsi.2024.9089 (DOI)